The CRISPR Journal Publishes Study Validating FAST-HDR Gene Editing Technology

ExpressCells

PHILADELPHIA, PA — ExpressCells, Inc., a biotechnology company that creates genetically edited cell lines for research, drug discovery, and production, announced that The CRISPR Journal recently published the first study that validates the company’s FAST-HDR technology.

This study, from Temple University, shows the benefits of using FAST-HDR to create genetically modified cell lines. This allowed the researchers to study three different proteins by tagging them with genes that code for fluorescent proteins. This removes burdensome work for detecting the proteins under study and accelerates the researchers’ ability to track them via live-cell imaging.

“As a research scientist, I grew frustrated with the long lead times and inflexibility of older methods to detect multiple proteins by microscopy that needed cell fixing and antibodies” said Dr. Oscar Perez Leal, Assistant Professor at the Temple University School of Pharmacy and co-founder of ExpressCells. “I invented the FAST-HDR technology and realized it would benefit other scientists interested in confocal microscopy without using antibodies or chemical staining. That is why we established ExpressCells.”

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The publication, “Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth,” outlines how the FAST-HDR vector system, used in combination with CRISPR-Cas9, allows visual live-cell studies of up to three endogenous proteins within the same cell line. The researchers developed gene-edited cellular models that allowed them to study physiological processes such as autophagy and mitochondrial dynamics in live cells. With these cells, they studied the role of multiple proteins from the virus that causes the COVID-19 disease and were able to identify the protein ORF3a as a potent inhibitor of autophagy, inducer of mitochondrial relocalization, and a growth inhibitor.

Dr Perez added “the FAST-HDR system leverages the cell’s own repair mechanism to insert DNA-encoded labeling tags into specific genes of the cell’s genome. This allows the creation of cell lines with precise labeling of cellular structures and drug targets much faster than previous methodologies”

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